Addgene

We narrowed to 13,636 results for: Egf

Showing: 8941 - 8970 of 13636 results

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCB19

Plasmid

#194757

Purpose

EGFP-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

EGFP-Tag

Expression

Mutation

none

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF16B tail 458-1317

Plasmid

#134624

Purpose

Mammalian expression of human KIF16B 458-1317

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF16B (KIF16B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 457 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (Y223F, Y248F)-mGFP

Plasmid

#180790

Purpose

Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1 (Y223F, Y248F) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

PD-1 (Y223F, Y248F)

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-HBO1-G485A pc2201

Plasmid

#167570

Purpose

The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

Depositor

Cristina Cardoso

Article

Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

Insert

histone acetyltransferase 1 (KAT7 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G485A

Promoter

CMV

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-LCa

Plasmid

#59353

Purpose

Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Clathrin Light Chain A (CLTA Human)

Use

Tags

EGFP and FKBP

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMVS102_P3-GFP-NATMX6

Plasmid

#99048

Purpose

EGFP reporter with six binding sites for the Zif268 DNA binding domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

McIsaac 2014 P3 promoter
eGFP
clonNAT resistance

Use

Tags

Expression

Yeast

Mutation

GAL1 promoter with 4 GAL4 sites removed and repla…

Promoter

McIsaac 2014 P3 promoter

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Short

Plasmid

#47394

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

Intersectin short (ITSN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞GFP

Plasmid

#154015

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin-S3A

Plasmid

#78296

Purpose

Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

Depositor

Michael Frotscher

Article

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3A

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-myrGFP

Plasmid

#190233

Purpose

AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

myristoylation signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-palGFP

Plasmid

#190232

Purpose

AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

palmitoylation signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1C tail 400-1103

Plasmid

#134621

Purpose

Mammalian expression of human KIF1C 400-1103

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1C (KIF1C Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74A)

Plasmid

#140731

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74A)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Expression

Mutation

Promoter

CamK II

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GalT-IRES-Puromycin

Plasmid

#134862

Purpose

Expresses EGFP-tagged Golgi marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

beta 1,4-galactosyltransferase (B4GALT1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 83-398

Promoter

EF1a

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 RD/LD

Plasmid

#186468

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

R404D/L406D (mutant unable to bind FFAT motifs)

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-GFP

Plasmid

#45171

Depositor

Hunt Willard, Brian Chadwick

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-NLS-CBGreen-FIRE-puro

Plasmid

#139195

Purpose

Luminescent reporter of ERK activity

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBG99

Use

Luciferase and Retroviral

Tags

Fra1 PEST domain fragment (FIRE) and nuclear loca…

Expression

Mammalian

Mutation

See depositor comments below

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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miniABEmax (pRZ900)

Plasmid

#131311

Purpose

CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Use

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
GFP-IP3KB

Plasmid

#32528

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KB (ITPKB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only