1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

   Plasmid

   #141025

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR-L1-PGK-Neo-L2

   Plasmid

   #141007

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SEPLuc-IRES-Antares

   Plasmid

   #141085

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEPLuc-EMCV IRES-Antares
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR-L1-PGK-PuroTK-L2

   Plasmid

   #141005

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   PuroTK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUAP1_Ta_Yr7_CDS_no_STOP

   Plasmid

   #141093

   Purpose

   Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

   Depositor

   Cristobal Uauy

   Article

   Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

   Insert

   Ta_Yr7
   

   Use

   Unspecified; Level 0 module for golden gate cloni…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-L3-Cas9-L2

   Plasmid

   #141038

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Antares-SEPLuc

   Plasmid

   #141086

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-EMCV IRES-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 66 to 91 on the Luciferas…

   Promoter

   CMV

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

   Plasmid

   #141052

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDESTFLAGHApuro3-Ago2Q545AK570A

   Plasmid

   #14105

   Depositor

   Thomas Tuschl

   Article

   Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

   Insert

   Ago2 (AGO2 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   changed Gln545 to Ala and Lys570 to Ala

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pDESTFLAGHApuro3-Ago2K570A

    Plasmid

    #14102

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Lys570 to Ala

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pDESTFLAGHApuro3-Ago2K533A

    Plasmid

    #14100

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Lys533 to Ala

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pDESTFLAGHApuro3-Ago2Q545A

    Plasmid

    #14101

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Gln545 to Ala

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pDESTFLAGHApuro3-Ago2K533AQ545A

    Plasmid

    #14103

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Lys533 to Ala, Gln545 to Alanine

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. fru-T2A-QF2 HDR plasmid

    Plasmid

    #141099

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SITS-ccdb

    Plasmid

    #141067

    Purpose

    Multisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBO

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-VASA-tdTomato-pNeo

    Plasmid

    #141030

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR2-L3-SfoI-Venus-PBL-R1

    Plasmid

    #141019

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-L1-U6-BsmBI-R5

    Plasmid

    #141032

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC-DEST-cjSTELLA-mCerulean-PuroTK

    Plasmid

    #141029

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR-P3P1r

    Plasmid

    #141014

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge