Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 188 results for: SAM activators

Showing: 41 - 60 of 188 results

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lentiSAMv2 C2CD4A/B-enh1-guide3

Plasmid

#125467

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide4

Plasmid

#118187

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide1

Plasmid

#118184

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide4

Plasmid

#118199

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 TCF7L2 TSS-guide3

Plasmid

#118186

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA-10Xcapture-PuroR

Plasmid

#211496

Purpose

Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

U6-gRNA
Ef1a-PuroR

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

Plasmid

#124541

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro

Expression

Mammalian

Mutation

WT, WT

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124539

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55A, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

Plasmid

#124537

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55G, d11-41 C55G

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EphA1-SAM-Cry2olig

Plasmid

#176600

Purpose

EphrinA1 RTK fusion to Cry2olig - includes SAM domain

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphrinA1-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pm-EphA1-SAM-Cry2olig

Plasmid

#176684

Purpose

membrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM domain

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

PM-EphA1-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 ZFAND3-guide2

Plasmid

#125514

Purpose

CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 ZFAND3-guide1

Plasmid

#125513

Purpose

CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs58692659-guide4

Plasmid

#125512

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs58692659-guide3

Plasmid

#125511

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs58692659-guide2

Plasmid

#125510

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs58692659-guide1

Plasmid

#125509

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs7732130-guide3

Plasmid

#125505

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 rs7732130-guide2

Plasmid

#125504

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7732130 (ZBED3 GWAS locus)

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 41 - 60 of 188 results

Results per page:

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