Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 114,144 results for: MPI

Showing: 5981 - 6000 of 114144 results

  1. pREP81xV5_ccdB

    Plasmid
    #79019
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79019-plasmid-map-sequence-id-151964

    Enlarge

  2. pREP82xV5_ccdB

    Plasmid
    #79020
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79020-plasmid-map-sequence-id-147777

    Enlarge

  3. Mouse NCKX2-AS-short (pcDNA3.1+)

    Plasmid
    #75198
    Purpose
    Mammalian expression of SLC24A2
    Depositor
    Jonathan Lytton
    Insert
    SLC24A2 (Slc24a2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    alternatively spliced exon absent
    Promoter
    CMV
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75198-plasmid-map-sequence-id-142840

    Enlarge

  4. pJK241

    Plasmid
    #71693
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Tro404 to Phe
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71693-plasmid-map-sequence-id-129669

    Enlarge

  5. pJK699

    Plasmid
    #71691
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Asp371 to Asn
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71691-plasmid-map-sequence-id-129671

    Enlarge

  6. pJK708

    Plasmid
    #71692
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Trp400 to Phe
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71692-plasmid-map-sequence-id-129670

    Enlarge

  7. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Tags
    Expression
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

    Enlarge

  8. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

    Enlarge

  9. PX458_ZNF3_1

    Plasmid
    #72363
    Purpose
    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72363-plasmid-map-sequence-id-132113

    Enlarge

  10. PX458_ZNF3_2

    Plasmid
    #72364
    Purpose
    Encodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72364-plasmid-map-sequence-id-134647

    Enlarge

  11. PX458_PBX2_1

    Plasmid
    #72357
    Purpose
    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72357-plasmid-map-sequence-id-132107

    Enlarge

  12. PX458_PBX2_2

    Plasmid
    #72358
    Purpose
    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72358-plasmid-map-sequence-id-132108

    Enlarge

  13. pJK539

    Plasmid
    #72515
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72515-plasmid-map-sequence-id-132543

    Enlarge

  14. pJK555

    Plasmid
    #72521
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72521-plasmid-map-sequence-id-132606

    Enlarge

  15. pJK577

    Plasmid
    #72528
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, small subunit
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72528-plasmid-map-sequence-id-132616

    Enlarge

  16. 5CBD

    Plasmid
    #69970
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. 5C4Q

    Plasmid
    #69963
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363130.1:MAC055-F03:C235029
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. psicheck_200_300_CA_repeat

    Plasmid
    #65880
    Purpose
    Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 200-300 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. psicheck_300_400_CA_repeat

    Plasmid
    #65879
    Purpose
    Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 300-400 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. MAC04E-A09

    Plasmid
    #58757
    Purpose
    bacterial expression of PDB 4O2Z
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4O2Z
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Sept. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5981 - 6000 of 114144 results