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AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159964

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159958

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159957

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159960

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159959

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159963

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13972 - pAAV-GfaABC1D-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE3-BGHpA (Alias: CN3972)

Plasmid

#208159

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AiP13798 - pAAV-DLX2.0-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN3798)

Plasmid

#229935

Purpose

DLX2.0 is an enhancer sequence, designed to drive cytosolic SYFP2 and nuclear mScarlet expression in forebrain GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2-P2A-mScarlet-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

Plasmid

#112261

Purpose

Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

spCas9-p300 (EP300 Human, Synthetic)

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Plasmid

#220613

Purpose

Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

Plasmid

#220608

Purpose

Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet

Use

AAV and Cre/Lox

Tags

3x HA; ArgiNLS

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

Plasmid

#220614

Purpose

Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet

Use

AAV and Cre/Lox

Tags

3x HA; ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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GFAP-MCS-WPRE-SV40 - GCH31

Plasmid

#230829

Purpose

AAV cloning vector for expression under the GFAP promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

Plasmid

#208111

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, scaffold

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAS133

Plasmid

#211504

Purpose

AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mTagBF2

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS134

Plasmid

#211505

Purpose

AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

Venus

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1⍺-DIO-Cx35-M1-mApple

Plasmid

#255635

Purpose

Encodes M. americana Cx35, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mApple tag. Mutation of K221E. Expression driven by EF1a promoter.

Depositor

Kafui Dzirasa

Article

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx35

Use

AAV

Tags

mApple

Mutation

K221E

Promoter

EF1a

Available Since

June 11, 2026

Availability

Academic Institutions and Nonprofits only

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