Addgene

We narrowed to 13,636 results for: Egf

Showing: 9061 - 9090 of 13636 results

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pCMV-dCas12a-ABE7

Plasmid

#193644

Purpose

Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA-TadA*7.10, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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SECURE miniABEmax-V82G (pJUL1828)

Plasmid

#131313

Purpose

CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-gfaABC1D-monSTIM1

Plasmid

#161797

Purpose

Lentiviral vector expressing monSTIM1 in astrocytes.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

gfaABC1D

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS010: pLX304-CheRiff-GFP

Plasmid

#62986

Purpose

Lentivirus production of CheRiff channelrhodopsin actuator

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral; Gateway destination vector

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B GG

Plasmid

#89451

Purpose

Expression of GFP-tagged human Rab27B GG in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B GG (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

GG (geranyl geranyl site is mutated)

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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SECURE miniABEmax-K20A/R21A (pJUL1774)

Plasmid

#131312

Purpose

CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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hA3A-BE3 (pRZ215)

Plasmid

#131314

Purpose

CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CamKIIa-monSTIM1

Plasmid

#161796

Purpose

Lentiviral vector expressing monSTIM1 in neurons.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CamKIIa

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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hAID-BE3 (pRZ352)

Plasmid

#131316

Purpose

CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSico Dnmt1

Plasmid

#12165

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

Dnmt1 shRNA (Dnmt1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

Plasmid

#108484

Purpose

inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
GFP-CRY2-P14P5K

Plasmid

#79569

Purpose

expression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c

Depositor

Olof Idevall-Hagren

Article

Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26

Insert

Pip5k1c (Pip5k1c Mouse)

Use

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

R445E and R446E; truncation of amino acids 636-66…

Promoter

CMV

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only
pAAV_Donor/Reporter-COL4A5

Plasmid

#130280

Purpose

COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_ACTB CRISPIE

Plasmid

#172853

Purpose

Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Inserts

Actb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
LP399

Plasmid

#90176

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-1289

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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LP696

Plasmid

#90177

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1289-1826

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1289-1826

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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LP344

Plasmid

#90174

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-370

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-370

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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LP241

Plasmid

#90173

Purpose

pEGFP-N1 with cDNA encoding HsKIF13B aa residues 1-557

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-557

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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LP346

Plasmid

#90175

Purpose

pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1000

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

KIF13B (KIF13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

aa residues 1-1000

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1210

Plasmid

#29107

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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PX4

Plasmid

#223340

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)

Use

Tags

EGFP-miniTurboID-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PX2

Plasmid

#223338

Purpose

Mapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

ABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)

Use

Tags

EGFP-TurboID-3HA

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MT2

Plasmid

#223330

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Article

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)

Use

Tags

3HA-TurboID-EGFP

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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