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Showing: 9121 - 9150 of 13641 results

  6. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87836-plasmid-map-sequence-id-176310

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  9. pLenti PGK Puro GFP-LC3B G120

    Plasmid
    #123241
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 121-125. Stop codon after G12…
    Promoter
    PGK
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123241-plasmid-map-sequence-id-245043

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  11. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166032-plasmid-map-sequence-id-338747

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  15. pCMV-RVR-ABE8e-V106W

    Plasmid
    #193659
    Purpose
    Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-TadA*8e-V106W, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193659-plasmid-map-sequence-id-422134

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  17. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid
    #123243
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acid 117. Stop codon after G116
    Promoter
    PGK
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123243-plasmid-map-sequence-id-244543

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  22. pLenti PGK Puro GFP-LC3B G120A

    Plasmid
    #123242
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    PGK
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123242-plasmid-map-sequence-id-244541

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  28. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

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  29. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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Showing: 9121 - 9150 of 13641 results