We narrowed to 8,451 results for: Ott

1. pcDNA5-FRT/TO-FLAG-SHLD2

   Plasmid

   #114117

   Purpose

   Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pPHAGE-CR-PINK1-C-TAP

   Plasmid

   #128510

   Purpose

   Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PTEN induced kinase 1 (PINK1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA tags

   Expression

   Mammalian

   Mutation

   Synonymous silent mutation in Q5 to render sgRNA …

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW-eGFP-FHA-SHLD2

   Plasmid

   #114121

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   RNF8 FHA domain (aa 2-160) and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pAAV-CMV-DIO-Magneto2.0-sNRPpA

   Plasmid

   #74307

   Purpose

   Cre-dependent expression of Magneto2.0 in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760-871

   Promoter

   CMV (after Cre recombination)

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

   Plasmid

   #52505

   Purpose

   mammalian expression vector of 53BP1 28A mutant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   53BP1 28A (TP53BP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   All 28 SQ/TQ motifs in N-term of 53BP1 have been …

   Promoter

   Available since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLEX_307-SOD1E133insTT

   Plasmid

   #83446

   Purpose

   Mammalian expression of SOD1E133insTT.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E133insTT (after GA)

   Promoter

   EF-1α

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

   Plasmid

   #197500

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW-eGFP-SHLD2-m1

   Plasmid

   #114120

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (m1 mutant) (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   W489A, F494A, W495A mutations introduced

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

   Plasmid

   #74302

   Purpose

   Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   Zebrafish

   Tags

   FLAG, cmcl2::EGFP, and p2A-mCherry

   Expression

   Mutation

   TRPV4: delta 760-871

   Promoter

   Neurog1

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW-eGFP-FHA

    Plasmid

    #114129

    Purpose

    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 2-160

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

    Plasmid

    #194015

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.1)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

    Plasmid

    #74306

    Purpose

    Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Inserts

    TRPV4 (Trpv4 Rat)
    ferritin (FTL and HTL1) (FTL, FTH1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (after cre recombination)

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGLUE-SHLD2

    Plasmid

    #114118

    Purpose

    Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Tags

    HA tag, Streptavidin-binding tag (Streptag), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX_307-SOD1E133Δ

    Plasmid

    #83445

    Purpose

    Mammalian expression of SOD1E133Δ.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133Δ

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLENTI-PGK-HYGRO-GATA6mut-HA

    Plasmid

    #65476

    Purpose

    Lentiviral expression of GATA6 Znc-finger mutant

    Depositor

    Eduard Batlle

    Article

    Whissell et al Nat Cell Biol. 2014 Jul;16(7):695-707. d

    Insert

    GATA6mut (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacking the eight most highly conserved bases of …

    Promoter

    PGK

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSOD1E133K-AcGFP1

    Plasmid

    #83442

    Purpose

    Expresses SOD1E133K in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133K

    Promoter

    CMV

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

    Plasmid

    #223118

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge