We narrowed to 10,729 results for: nar;

1. pMJ915v2+Nterm Spe1 +Cterm Age1 site

   Plasmid

   #88914

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

   Plasmid

   #22930

   Purpose

   Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

   Depositor

   Alexandra Newton

   Article

   Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

   Insert

   PHLPP1-deltaPH (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes 127-1205 aa. Deleted amino-terminal PH d…

   Promoter

   CMV

   Available since

   Feb. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pG3B-U3Ub

   Plasmid

   #134750

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b-mLin28A-cHIS

   Plasmid

   #137998

   Purpose

   Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

   Depositor

   Judy Lieberman

   Article

   O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

   Insert

   mouse Lin28A, CSD and ZnFs (residues 36–186)
   

   Use

   Tags

   C-terminal His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK1003 (pSL2)

   Plasmid

   #190998

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-RP2

   Plasmid

   #86072

   Purpose

   A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   RP2 (RP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-attBr-ZsGreen-attP

   Plasmid

   #51265

   Purpose

   Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-ZsGreen-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETCON-TNF

   Plasmid

   #67748

   Purpose

   Tumor Necrosis Factor Alpha Yeast Display Plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

   Insert

   TNF-Alpha
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GL2 shRNA

   Plasmid

   #86083

   Purpose

   A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   control shRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP SECFP-Stx17TM

    Plasmid

    #86777

    Purpose

    Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    transmembrane domains of human Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOAD

    Plasmid

    #196825

    Purpose

    Yeast two-hybrid ; Gal4 activation domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL1.7XX

    Plasmid

    #49344

    Purpose

    luciferase reporter with mouse Bmp2 promoter

    Depositor

    Melissa Rogers

    Article

    Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

    Insert

    Bmp2 distal promoter (Bmp2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    nt –1237 to +471

    Promoter

    Bmp2 distal promoter

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. hP14Gal4

    Plasmid

    #84364

    Purpose

    Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    Gal4 and HA tag

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CMV/TO

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-mROCK1-delta4

    Plasmid

    #101292

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    76-1354 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

    Plasmid

    #159487

    Purpose

    Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

    Depositor

    Julius Lucks

    Article

    Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

    Insert

    Termination Module:Linear-2_PolyU(1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-27

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-sgRbm10/Cre

    Plasmid

    #89648

    Purpose

    Expresses an Rbm10-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rbm10 (Rbm10 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-mROCK1-delta6

    Plasmid

    #101294

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    375-1354 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pChlr-8CQ

    Plasmid

    #52171

    Purpose

    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed 12N to 12C in module 8

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Lenti-sgSmad4/Cre

    Plasmid

    #89651

    Purpose

    Expresses a Smad4-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Smad4 (Smad4 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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