We narrowed to 8,311 results for: Dos
-
Plasmid#217756PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
TagsEGFPExpressionMammalianMutationTwo tandem CA3 domain (aa 317-400) 1st linker GG…PromoterCMVAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSR1-CA3-GS-mRFP1 (C-KSR-GS)
Plasmid#217758PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
TagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 with GGSSGGGGA linkerPromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressPromoterhuman Synapsin 1 (hSyn)Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-AnkG ZU5in-3xHA
Plasmid#213730PurposeMammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.DepositorInsertAnkG ZU5in (Ank3 Mouse)
ExpressionMammalianAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-AnkB ZU5ex-3xHA
Plasmid#213732PurposeMammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.DepositorInsertAnkB ZU5ex (Ank2 Mouse)
ExpressionMammalianAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-AnkG ZU5ex-3xHA
Plasmid#213731PurposeMammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.DepositorInsertAnkG ZU5ex (Ank3 Mouse)
ExpressionMammalianAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
INS-3'gRNA
Plasmid#210468PurposegRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertINS (INS Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 pCAGGS
Plasmid#206101Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q)
Plasmid#200979PurposeMammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
TagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q mutation fo…PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV R354H SPOP 28-359
Plasmid#197011PurposeE. coli expression vectorDepositorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro
Plasmid#192131PurposeExpresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance geneDepositorInsertHsp1-Hsp2Cas9
UseAAVExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro
Plasmid#192132PurposeExpresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance geneDepositorInsertHsp1-Hsp2Cas9-Y
UseAAVExpressionMammalianMutationHsp1-Hsp2Cas9 (Y446A)Available SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Staphylococcus aureus, Human)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (HLA-A Staphylococcus aureus, Human)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-ND-Cdt1-mCitrine
Plasmid#193771PurposeHuman Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCitrine (CDT1 Human)
TagsmCitrineExpressionMammalianMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background) (CYP8B1 Staphylococcus aureus, Human)
Tagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin-S796A
Plasmid#186434PurposeExpresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
TagsmCherryExpressionMammalianMutationChanged Serine 796 to AlaninePromoterCMV IE94Available SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-14
Plasmid#160714PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)
UseSynthetic BiologyAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only