We narrowed to 59,208 results for: Ran

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pCMV-HA-PGRMC1-21-195

Plasmid

#173473

Purpose

PGRMC1 lacking the N-terminal region upstream of the transmembrane region

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1 (PGRMC1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deleted for amino acids 1-20

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAraQCas-BsaI

Plasmid

#170633

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO chicken-cPLA2-C2-domain

Plasmid

#170753

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Chicken)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2082

Plasmid

#155162

Purpose

Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaFΔ[248-269])-(6xHis-TEV-MlaB)

Expression

Bacterial

Mutation

deleted amino acids 248-269 in MlaF

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1965

Plasmid

#155152

Purpose

Expresses MlaF(1-246)-(6 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1966

Plasmid

#155153

Purpose

Expresses MlaF(1-246)-(10 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1967

Plasmid

#155154

Purpose

Expresses MlaF(1-246)-(14 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N0

Plasmid

#160983

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1-2A

Plasmid

#160986

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2-2A

Plasmid

#160987

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2

Plasmid

#160985

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1

Plasmid

#160984

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-JNKKTR -miRFP670-WPRE

Plasmid

#159976

Purpose

single wavelength translocating reporter of the JNK pathway

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

JNKKTR -miRFP670

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Expression

Bacterial

Mutation

V124N

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynNanobody IRES EGFP

Plasmid

#136624

Purpose

Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK224

Plasmid

#110590

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTH825-CEN-minRLuc/slowstaCFLuc

Plasmid

#115370

Purpose

Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase

Depositor

Tobias von der Haar

Inserts

Gcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)

Expression

Yeast

Mutation

All codons have been changed for the least favour…

Promoter

ADH1 and TDH3

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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