We narrowed to 9,173 results for: Pol

1. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Expression

   Bacterial

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-DTX2-1-178-EGFP-Hygro

   Plasmid

   #238094

   Purpose

   Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   DTX2 (DTX2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-178 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CDH5-mRuby3-IRES-puro

   Plasmid

   #85144

   Purpose

   lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Promoter

   CAG

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET32a-HD25Q

   Plasmid

   #11508

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 25 glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 25 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

   Plasmid

   #169186

   Purpose

   To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
   

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1-CAT

   Plasmid

   #173943

   Purpose

   Bacterial expression of isolated PARP1 CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_GR-RE_MLPmin_BC1092-luc2

   Plasmid

   #227112

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

   Plasmid

   #127177

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Promoter

   None

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-hCdt1-HA

   Plasmid

   #117500

   Purpose

   Expresses human Cdt1 fused to HA tag

   Depositor

   Domenico Maiorano

   Article

   Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

   Insert

   Cdc10-dependent transcript-1 (CDT1 Human, 1700 bp)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmirGLO-3'UTR-TNF

    Plasmid

    #153069

    Purpose

    Human TNF 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of TNF gene (TNF Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-Zn2

    Plasmid

    #174805

    Purpose

    Bacterial expression of isolated PARP1 Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn2 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1-Zn3

    Plasmid

    #174806

    Purpose

    Bacterial expression of isolated PARP1 Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn3 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-His10FLAG

    Plasmid

    #157736

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Tags

    His10 + 3xFLAG, enterokinase-cleavable

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Frt-V5-hspB8

    Plasmid

    #63109

    Purpose

    Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCWXPG-UBI-Ngn3PDX1MafA

    Plasmid

    #114303

    Purpose

    Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    Ngn3 (NEUROG3 Human)
    

    Use

    Lentiviral

    Mutation

    Ngn3, PDX1 and MafA proteins are expressed from a…

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1 DOX off blast NFS1

    Plasmid

    #160801

    Purpose

    Express Dox repressible NFS1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET32a-HD16Q

    Plasmid

    #11487

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 16 glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 16 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

20. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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