We narrowed to 15,724 results for: LEA

1. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-Ef1a-Coff/Fon-EYFP

   Plasmid

   #231927

   Purpose

   Cre-off/Flp-on EYFP under the Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-DsRed_IRES_SNCA:EGFP

   Plasmid

   #92195

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-SNCA-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-EF1a-CCO-IRES-Puromycin

   Plasmid

   #134861

   Purpose

   Expresses EGFP-tagged inner mitochondrial membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome c oxidase subunit VIII (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-CAG-SPOTlight-U2GCR

   Plasmid

   #164819

   Purpose

   Reporter to measure ISR state-dependent protein synthesis

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   SPOTlight-U2GCR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGPTVII-nlsSED1

   Plasmid

   #178190

   Purpose

   SED1 biosensor for expression in Arabidopsis thaliana

   Depositor

   Jose Dinneny, Cesar Cuevas-Velazquez

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

   Plasmid

   #182651

   Purpose

   human CD81-TEV cleavage site-FKBP-SmBiT tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)
   

   Tags

   SmBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

    Plasmid

    #182926

    Purpose

    Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Type

    Empty backbone

    Tags

    APEX2 and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pShuttle-Flag-XBP1u

    Plasmid

    #63679

    Purpose

    Adenoviral shuttle vector for XBP1u expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    CMV

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

    Plasmid

    #230935

    Purpose

    gRNA cloning vector encoding BFP and puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Promoter

    hU6

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

    Plasmid

    #187588

    Purpose

    Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NLS
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-Flpo

    Plasmid

    #55637

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV2

    Insert

    Flpo
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-CRISPRv2-sgNTC13-hygro

    Plasmid

    #231988

    Purpose

    Knockout non-targeting control

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nLuc_AP4_TEVs_P3mS

    Plasmid

    #119299

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    

    Use

    Luciferase

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 4_T7-mScarlet3-RitC

    Plasmid

    #225931

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-RitC
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG_iPE-N

    Plasmid

    #214734

    Purpose

    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-N_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-actin

    Plasmid

    #118378

    Purpose

    Gateway compatible donor vector with WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge