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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MsmegA _pET29b

Plasmid

#215411

Purpose

Expression construct for MsmegA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MsmegA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR-hCD2

Plasmid

#135392

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

gRNA_hIRF1 promoter #12

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-YPet

Plasmid

#215747

Purpose

Expresses MS2 coat protein (MCP) fused to YPet

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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