We narrowed to 8,554 results for: sgRNA

1. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR_Puro_sgTP53

   Plasmid

   #187819

   Purpose

   pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgTP53
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelp

   Plasmid

   #227583

   Purpose

   Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   guide RNA scaffold
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCP-tRNA

   Plasmid

   #133812

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT07

   Plasmid

   #223379

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOsU3

   Plasmid

   #170132

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459V2.0-HypaCas9-mRuby2

   Plasmid

   #183872

   Purpose

   pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HypaCas9-P2A-mRuby2-T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR-V2_mMSH2

   Plasmid

   #186156

   Purpose

   sgRNA targeting murine Msh2 gene

   Depositor

   David McFadden

   Article

   Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

   Insert

   Msh2_gRNA (Msh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgTrack-mCherry

    Plasmid

    #114013

    Purpose

    Empty sgRNA expression vector with co-expression of mCherry reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiGuide_mCherry_NT135

    Plasmid

    #183121

    Purpose

    pLentiGuide modified to express mCherry and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide_CjCas9-Puro

    Plasmid

    #202564

    Purpose

    hU6-BsmBI-gRNA-Puro

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    CjCas9 sgRNA cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Expression

    Mutation

    Promoter

    SP30

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTJV1Sc-rpoB

    Plasmid

    #166978

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    Ec86 Retron (ECD_00831 )
    lambda bet
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101 and VanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR1068

    Plasmid

    #111092

    Purpose

    BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BlpI site disrupted

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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