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pGFP-mVprBP pc2953

Plasmid

#201698

Purpose

Expresses of GFP-tagged VprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pmcherry-mVprBP pc2954

Plasmid

#201699

Purpose

Expresses of mcherry-tagged mVprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCENPBR pc861

Plasmid

#167569

Purpose

Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

Homo sapiens centromere protein B (CENPB Human)

Tags

Ds-Red

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2miRFP670 pc3942

Plasmid

#167568

Purpose

The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

Depositor

Cristina Cardoso

Article

Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

Insert

MeCp2 (Mecp2 Rat)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCF153_Gag-Pol

Plasmid

#225961

Purpose

CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-GagPol (FMLV)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCF142_U6-sgRNA

Plasmid

#225960

Purpose

U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

U6-sgRNA (recipient)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT-B-GRPA

Plasmid

#226222

Purpose

Human RPA34 tagged to GFP under a EF1alpha promotor for generating stable HeLa Kyoto GFP-RPA34 using the Flp-In recombination system

Depositor

Cristina Cardoso

Article

Pabba et al Elife. 2023 Oct 31;12:RP87572. doi: 10.7

Insert

RPA34 (RPA2 Human)

Expression

Mammalian

Promoter

EF1ALPHA

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s pc3901

Plasmid

#201700

Purpose

Expresses of GFP-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEN-CAG-mRFP-PCNA pc2729

Plasmid

#166040

Purpose

Expresses mRFP tagged human PCNA in mammalian cells.

Depositor

Cristina Cardoso

Article

Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-APPL1-R146A/K152A/R154A

Plasmid

#59767

Purpose

Expresses APPL1 Endosomal Localization Mutant

Depositor

Donna Webb

Article

Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

Insert

APPL1 (APPL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R146A/K152A/R154A

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pTYB1-MBD2∆N∆CC (pc5082)

Plasmid

#232745

Purpose

Bacterial expression plasmid of MBD2-∆N∆CC. MBDTRD and C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2 lacking the C-terminus (a 236-414)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGmMBD1.4 (pc3305)

Plasmid

#246851

Purpose

Mammalian expression plasmid of mouse MBD1 lacking the MBD domain (aa 75–636). N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

Insert

MBD1 (Mbd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Mbd1ΔMBD, aa 75–636

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFRT-B-RPCNA (pc1205)

Plasmid

#247000

Purpose

This pFRT-B plasmid enables the creation of a stable mammalian cell line expressing RFP-tagged PCNA .

Depositor

Cristina Cardoso

Article

Chagin et al Nat Commun. 2016 Apr 7;7:11231. doi: 10.

Insert

PCNA (PCNA Human)

Use

Cre/Lox

Tags

RFP

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFRT-B-GPCNA (pc1274)

Plasmid

#247001

Purpose

This pFRT-B plasmid enables the creation of a stable mammalian cell line expressing GFP-tagged PCNA

Depositor

Cristina Cardoso

Article

Chagin et al Nat Commun. 2016 Apr 7;7:11231. doi: 10.

Insert

PCNA (PCNA Human)

Use

Cre/Lox

Tags

GFP

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG-MeCP2_R270X (pc4751)

Plasmid

#248579

Purpose

Mammalian expression plasmid of MeCP2-R294X (aa1- aa293)

Depositor

Cristina Cardoso

Article

Romero et al Commun Biol. 2025 Dec 8. doi: 10.1038/s4

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa1-aa269

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEG-MeCP2_R294X (pc4753)

Plasmid

#248580

Purpose

Mammalian expression plasmid of MeCP2-R294X (aa1- aa293)

Depositor

Cristina Cardoso

Article

Romero et al Commun Biol. 2025 Dec 8. doi: 10.1038/s4

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa1-aa293

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2 (pc4741 )

Plasmid

#249083

Purpose

Bacteria expression plasmid of GFP-MeCP2

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB1-MBD2∆C∆GR pc5079

Plasmid

#229759

Purpose

Bacterial expression plasmid of MBD2∆C∆GR. MBD2 lacking the C-terminus of MBD2: aa 236-414 and the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the C-terminus of MBD2: aa 236-414 a…

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB1-MBD2-C∆CC pc5084

Plasmid

#229761

Purpose

Bacterial expression plasmid of MBD2-C∆CC. C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

C-terminus of MBD2 is lacking the coiled coil dom…

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pmMBD2-N∆GR-G pc5087

Plasmid

#229763

Purpose

Mammalian expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

N-terminus of MBD2 lacking the glycine/arginine r…

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge