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pUPD3_PARTPRM_J23114

Plasmid

#243943

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23114

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23115

Plasmid

#243944

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23115

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23116

Plasmid

#243945

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23116

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23117

Plasmid

#243946

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23117

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23100

Plasmid

#243930

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23100

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23101

Plasmid

#243931

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23101

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23102

Plasmid

#243932

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23102

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_J23103

Plasmid

#243933

Purpose

GoldenBraid2.0 Constitutive promoter, lacking ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

J23103

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_Pem7

Plasmid

#243908

Purpose

GoldenBraid2.0 Strong, constitutive promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Pem7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_Pem7

Plasmid

#243919

Purpose

GoldenBraid2.0 Strong, constitutive promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Pem7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_Stuffer1

Plasmid

#243844

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_Stuffer2

Plasmid

#243847

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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POC1343

Plasmid

#221568

Purpose

Plasmid containing the Insert 1 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1344

Plasmid

#221569

Purpose

Plasmid containing the Insert 2 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1345

Plasmid

#221570

Purpose

Plasmid containing the Insert 3 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1346

Plasmid

#221571

Purpose

Plasmid containing the Insert 4 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB U6

Plasmid

#229956

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX458 ACTB

Plasmid

#229975

Purpose

ecnodes for spCas9 and sgRNA targeting last intron of ActinB gene

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBsgRNA and spCas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMpGWB306-MpRTN1

Plasmid

#228476

Purpose

Binary vector for the expression of MpRTN1 in plants

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-AePHOT-LOV1

Plasmid

#220113

Purpose

The expression of MBP fused to AePHOT-LOV1 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-LOV1

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-MpPHOT-LOV1

Plasmid

#220115

Purpose

The expression of MBP fused to MpPHOT-LOV1 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-LOV1

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AePHOT-Citrine

Plasmid

#220105

Purpose

Transient expression of AePHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-Citrine

Expression

Plant

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-AePHOT-LOV2

Plasmid

#220114

Purpose

The expression of MBP fused to AePHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-LOV2

Expression

Bacterial

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKM596-MpPHOT-LOV2

Plasmid

#220116

Purpose

The expression of MBP fused to MpPHOT-LOV2 in E. coli

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-LOV2

Expression

Bacterial

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Bneu-9

Plasmid

#205214

Purpose

Unit Bactoneuron-9, expresses EGFP

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHC_O2

Plasmid

#205224

Purpose

part of Bactoneuron DF2, expresses mvenus

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mVenus

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC_O3

Plasmid

#205225

Purpose

part of Bactoneuron DF4, expresses mTagBFP2

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mTagBFP2

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAC_O3

Plasmid

#205226

Purpose

part of Bactoneuron DF5, expresses mTagBFP2 and LuxR

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mTagBFP2, LuxR

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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