We narrowed to 13,665 results for: Cre

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Sema6a.a-AP-His

Plasmid

#72037

Purpose

Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.a (Sema6a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 DN-xIGFR

Plasmid

#24699

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

Insert

Dominant negative xIGFR (igf1r Frog)

Expression

Mammalian

Mutation

C-terminal truncated version of the Xenopus IGF t…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
LB360 (Bcl-2 from -1287 to -1337)

Plasmid

#15242

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1337 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/DH* (L213Q) uGFP

Plasmid

#14559

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174)/DH* (L213Q)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

Plasmid

#170226

Purpose

Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

IKBKB (IKBKB Human)

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD3-Strep-HA

Plasmid

#113488

Purpose

Expression of human UBXD3 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD3 (UBXN10 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBXD4-Strep-HA

Plasmid

#113480

Purpose

Expression of human UBXD4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBXD4 (UBXN2A Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5

Plasmid

#105509

Purpose

MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Inserts

Foxa1 (Foxa1 Mouse)
Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag and V5

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1318

Plasmid

#78587

Purpose

Expression flag-USP19 494-1318 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1318 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
Sema3a(S)-Fc-His

Plasmid

#72138

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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PFD1130w-bio-His

Plasmid

#50823

Purpose

Expresses enzymatically monobiotinylated full length PFD1130w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFD1130w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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RAP2-bio

Plasmid

#47795

Purpose

Expresses enzymatically monobiotinylated full-length RAP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RAP2

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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RON6-bio

Plasmid

#47785

Purpose

Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RON6

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin replaced…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG-FRA1

Plasmid

#188742

Purpose

expresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) for the dTAG system

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

FKBP F36V tag (dTAG)

Expression

Mammalian

Promoter

hPGK promoter

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PRMT2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176953

Purpose

Mammalian expression vector encoding PRMT2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

PRMT2 (PRMT2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP2A

Plasmid

#128349

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP2A (PTPA Human)

Tags

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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MoFGFR3b-SV40PA

Plasmid

#86493

Purpose

mamalian cell expression of FGFR3b

Depositor

David Ornitz

Article

Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

Insert

FgfrR3 IIIb 12F5 (Fgfr3 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT2A

Plasmid

#53109

Purpose

Expression of Drosophila 5-HT2A

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2A (5-HT2 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only