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TetO-FUW-SOX3

Plasmid

#61546

Purpose

Expresses SOX3 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

Plasmid

#211767

Purpose

dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pGK and pEF1a

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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mouse CENP-B-EGFP

Plasmid

#107264

Purpose

mouse CENP-B-EGFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

CENP-B-EGFP (Cenpb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

T7

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter + CNS-5

Plasmid

#20128

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGLACTE-PseudoHT51

Plasmid

#99257

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Synthetic, Human)

Mutation

Contains synthetic, non-human, primer sequence do…

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hST3GAL6-RFP

Plasmid

#22923

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

Plasmid

#214977

Purpose

Flag tagged LDS mutant LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B_mut (MAP1LC3B Human)

Expression

Mammalian

Mutation

LDS mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107320

Purpose

Expresses SpCas9 fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMX IkB alpha M

Plasmid

#12329

Depositor

Inder Verma

Article

Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Insert

IkB alpha M (Nfkbia Mouse)

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Expression

Mammalian

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

Plasmid

#199456

Purpose

Expression of stdPCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shSOD1

Plasmid

#102975

Purpose

Suppression of SOD1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shSOD1 (SOD1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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12x PRE TK luc

Plasmid

#206163

Purpose

Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

12X PRE TK luciferase

Use

Luciferase

Expression

Mammalian

Promoter

min TK

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23B

Plasmid

#201459

Purpose

Expresses human RAD23B with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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