We narrowed to 24,077 results for: Spr

1. EXOSC10 gRNA (BRDN0001149316)

   Plasmid

   #77990

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EXOSC10

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EXOSC10 (EXOSC10 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

   Plasmid

   #99690

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MINK1 gRNA (BRDN0001146260)

   Plasmid

   #76260

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MINK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MINK1 (MINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO5d.EFS.SpCas9.P2A.BSD

   Plasmid

   #57821

   Purpose

   Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   EFS (EEF1A1 Human)
   P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-SpG-His

   Plasmid

   #179318

   Purpose

   Plasmid for bacterial expression and purification of SpG-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpG
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DYRK1A gRNA (BRDN0001145031)

   Plasmid

   #76755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DYRK1A

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EFS-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222497

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FXN gRNA (BRDN0001144784)

   Plasmid

   #77813

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FXN

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FXN (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FXN gRNA (BRDN0001145648)

    Plasmid

    #77814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FXN

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FXN (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FXN gRNA (BRDN0001146360)

    Plasmid

    #77815

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FXN

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FXN (FXN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN, U6

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PINK1 gRNA (BRDN0001147554)

    Plasmid

    #78035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PINK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PINK1 (PINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PINK1 gRNA (BRDN0001145357)

    Plasmid

    #78036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PINK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PINK1 (PINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DGKQ gRNA (BRDN0001162275)

    Plasmid

    #77377

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKQ

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKQ (DGKQ Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAP3K3 gRNA (BRDN0001148097)

    Plasmid

    #76016

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K3 (MAP3K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ERN1 gRNA (BRDN0001162231)

    Plasmid

    #76409

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERN1 (ERN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SRC gRNA (BRDN0001145402)

    Plasmid

    #75650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SRC gRNA (BRDN0001149457)

    Plasmid

    #75652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SRC gRNA (BRDN0001146185)

    Plasmid

    #75651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SRC

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SRC (SRC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge