We narrowed to 7,281 results for: Tet-on

1. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

   Plasmid

   #178790

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

   Insert

   Pink Flamindo
   

   Use

   AAV

   Promoter

   hGFAP

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

   Plasmid

   #166609

   Purpose

   Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 3xFlag IFIT5

   Plasmid

   #53556

   Purpose

   mammalian expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMRE165

   Plasmid

   #118513

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT222

   Plasmid

   #40942

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD5-H5_Vietnam_1203/04_sfGFP_ST

   Plasmid

   #182546

   Purpose

   Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

   Depositor

   Robert de Vries

   Article

   Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

   Insert

   H5_Vietnam_1203_04
   

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAXEF-Rap1-E63(GTP)

   Plasmid

   #32698

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Expression

   Mammalian

   Mutation

   E63: Consitutively GTP-bound

   Promoter

   EF promoter

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Tags

   3x-FLAG

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Incoherent feedforward (IFF) plasmid

   Plasmid

   #169748

   Purpose

   Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-tight2-Tmem98-FLAG

    Plasmid

    #124506

    Purpose

    Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Tmem98 (Tmem98 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    tetO-CMV

    Available Since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE161

    Plasmid

    #118509

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-BaLgp140-TM

    Plasmid

    #111843

    Purpose

    Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Tags

    CD5 leader sequence and TM helix from MHC class I

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGK+ plasmid with onboard mCherry cassette

    Plasmid

    #169745

    Purpose

    PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175019

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSW002-PpsbA-DsRed-Express2

    Plasmid

    #111257

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    PpsbA

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE164

    Plasmid

    #118512

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSW002-Pc-DsRed-Express2

    Plasmid

    #111251

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. NMD2_lenti

    Plasmid

    #216221

    Purpose

    Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_b-globin intron x2
    

    Expression

    Mammalian

    Available Since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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