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pT7-EGFP-C1-HsXRN1_1630-1690_P

Plasmid

#147249

Purpose

Mammalian Expression of HsXRN1_1630-1690

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

HsXRN1_1630-1690 (XRN1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3C DD 616-796

Plasmid

#193938

Purpose

Mammalian expression of Halo-tagged Kif 3C DD 616-796

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3C (Kif3c Mouse)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3C DD 616-796

Plasmid

#193937

Purpose

Mammalian expression of GFP-tagged Kif 3C DD 616-796

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3C (Kif3c Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3C CC 411-615

Plasmid

#193935

Purpose

Mammalian expression of GFP-tagged Kif 3C CC 411-615

Depositor

Marvin Bentley

Publication

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3C (Kif3c Mouse)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_mCh-2xFKBP (pBS1157)

Plasmid

#185330

Purpose

For the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I

Expression

Mammalian

Mutation

D125I

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)

Plasmid

#185320

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120)

Plasmid

#185315

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC_98-130

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)

Plasmid

#185311

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-SspB (pBS1070)

Plasmid

#185293

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-dEgf

Plasmid

#173840

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

Plasmid

#168772

Purpose

Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

Depositor

Noboru Mizushima

Publication

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls (plaat1 Zebrafish)

Tags

EGFP

Promoter

alphaA-crystallin promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_A893E-talin1 (1-2541)

Plasmid

#166116

Purpose

For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

A893E-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

A893E

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L1539P-talin1 (1-2541)

Plasmid

#166119

Purpose

For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L1539P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L1539P

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L2509P-talin1 (1-2541)

Plasmid

#166123

Purpose

For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L2509P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L2509P

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_S1750F-talin1 (1-2541)

Plasmid

#166120

Purpose

For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

S1750F-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

S1750F

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2086V-talin1 (1-2541)

Plasmid

#166122

Purpose

For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

D2086V-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

D2086V

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_Y1389C-talin1 (1-2541)

Plasmid

#166118

Purpose

For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Publication

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

Y1389C-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Y1389C

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP2_SH3

Plasmid

#110016

Purpose

Protein expression and purification of GRAP2_SH3

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GRAP2_SH3 (GRAP2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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