We narrowed to 15,933 results for: nans

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GB3273

Plasmid

#193135

Purpose

A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1e.1

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Tags

His-MBP

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mouse KANK4-Y801H Ankyrin Repeats pET151

Plasmid

#164058

Purpose

Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

Depositor

Ben Goult

Insert

Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG1

Plasmid

#188967

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtggaaaacaatgcgaccgactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST tol2 UbB polyA

Plasmid

#188701

Purpose

Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSub-Asp6-MBP-HRas

Plasmid

#185758

Purpose

Bacterial expression of Asp6-MBP-HRas

Depositor

Jeffrey Bode

Article

Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

Insert

Asp6-MBP-HRas

Tags

MBP

Expression

Bacterial

Mutation

6x Asp added to the N terminus of MBP

Promoter

tac

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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Antares2-N1

Plasmid

#120869

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCS105

Plasmid

#174830

Purpose

Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

Depositor

Carol Greider

Article

Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

Insert

11R Unique

Tags

GAL4 5XUAS

Expression

Bacterial

Mutation

Changing TEL11R to a unique end

Promoter

lac promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585-gp130

Plasmid

#174404

Purpose

Bacterial expression of sarA-gp130 chimera under native promoter.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Expression

Bacterial

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0nt

Plasmid

#166993

Purpose

Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0), Hairpin = 0 nt

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4)

Plasmid

#166989

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4)

Plasmid

#166985

Purpose

Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt

Plasmid

#166995

Purpose

Tests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP1

Plasmid

#113972

Purpose

Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP2

Plasmid

#113973

Purpose

Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgiRFP3

Plasmid

#113974

Purpose

Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTalpha-FliG

Plasmid

#154361

Purpose

Bacterial expression of Mtalpha

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Mtalpha

Expression

Bacterial

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTn-FliG

Plasmid

#154360

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159941

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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