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pBabe-puro-miR-10b sponge

Plasmid

#25043

Depositor

Bob Weinberg

Article

Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

Insert

miR-10b sponge

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
P-del (pTransluc-miR-145-2)

Plasmid

#21500

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

0.9 kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pZS*11-yfp0

Plasmid

#53241

Purpose

express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

Depositor

Philippe Cluzel

Article

Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtetO1

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAM5187

Plasmid

#120086

Purpose

Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.

Depositor

Susan Golden

Article

Chen et al Microbiology. 2016 Dec;162(12):2029-2041

Type

Empty backbone

Use

Shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTOM40.2

Plasmid

#89882

Purpose

Expresses shRNA against TOM40

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCM3.06

Plasmid

#19602

Depositor

Geraldine Seydoux

Article

Lee et al PLoS Genet. 2007 Dec 28. 3(12):e233.

Insert

fbf-1 ORF+3' in pDONR201 (fbf-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBSU6-shTOM40.3

Plasmid

#89796

Purpose

Expresses shRNA against TOM40.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSIN-LexAOx4-Pmin-mCherry

Plasmid

#192633

Purpose

Lentiviral vector for inducible mCherry expression.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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KHT-1

Plasmid

#113054

Purpose

Expresses worm KHT-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al EMBO J. 2009 Jun 3;28(11):1601-11. doi:

Insert

K+ channel for Habituation to Tap subunit 1

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p15a-AsCas12f-blaZ

Plasmid

#171609

Purpose

AsCas12f1-based E. coli genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQAT03

Plasmid

#223375

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT05

Plasmid

#223377

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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TrkB Acceptor

Plasmid

#83953

Purpose

FRET acceptor for TrkB mEGFP

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Phospholipase C gamma 1 (Plcg1 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

Plasmid

#159899

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNAv1-empty

Plasmid

#171611

Purpose

sgRNA_v1 plasmid for AsCas12f1 in bacteria

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1_sgRNA_1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p15a-AsCas12f-apmR

Plasmid

#171610

Purpose

AsCas12f1-based K. pneumoniae genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NGFP

Plasmid

#52734

Purpose

IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

NGFP+Z-fusion

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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