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Plasmid#219414PurposeEstradiol-inducible expression of 13-6 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4
Plasmid#47875PurposeDestination plasmid with mRuby2 for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK1056
Plasmid#209546PurposeBTK Type 4 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 4 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTALYM9
Plasmid#47876PurposeDestination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsTy1tagExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK1052
Plasmid#209542PurposeBTK Type 2 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 2 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTALYM3
Plasmid#47874PurposeDestination plasmid with mClover for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmCloverExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYY62-P1int-TP901-GFPdropout
Plasmid#235773PurposePlasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1053
Plasmid#209543PurposeBTK Type 3-4 fluorescent protein cassette for Golden Gate assemblyDepositorInsertType 3-4 E2-Crimson cassette
UseSynthetic BiologyTagsExpressionMutationPromoterCP25Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRW402-P1int-BXB1
Plasmid#235979PurposePlasmid used for constructing donor DNA for BXB1-compatible p1 integration. You can insert your GOIs into this plasmid by using Golden Gate Assembly.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F4
Plasmid#195718PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#4 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F20
Plasmid#195734PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#20 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F5
Plasmid#195719PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#5 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F12
Plasmid#195726PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#12 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F3
Plasmid#195717PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#3 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F24
Plasmid#195738PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#24 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F7
Plasmid#195721PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#7 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F8
Plasmid#195722PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#8 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F14
Plasmid#195728PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#14 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits