We narrowed to 1,807 results for: tet on vector

1. pCBLsgRNA7

   Plasmid

   #199733

   Purpose

   Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU6.2-sgRNA7 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBLsgRNA8

   Plasmid

   #199734

   Purpose

   Golden Gate entry vector; 8th gRNA under TaU6 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   TaU6-sgRNA8 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBacMam8_N_Flag_3C

   Plasmid

   #178818

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBacMam2_N_His8_eGFP_TEV

   Plasmid

   #178806

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   His8, eGFP, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBacMam3_N_His8_eGFP_3C

   Plasmid

   #178807

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBacMam4_N_Flag_His8_eGFP_TEV

   Plasmid

   #178808

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, His8, eGFP, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBacMam7_N_Flag_TEV

   Plasmid

   #178817

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBacMam5_N_Flag_His8_eGFP_3C

   Plasmid

   #178816

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBacMam6_C_3C_His8_Stop

   Plasmid

   #178810

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   3C, His8, Stop codon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LT3GEPIR-MSH2shRNA1

    Plasmid

    #220587

    Purpose

    To inducibly knockdown MSH2 expression

    Depositor

    Sean Lee

    Insert

    MSH2 shRNA1 (MSH2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE TRE dCas9-VP64

    Plasmid

    #50916

    Purpose

    Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A nuclease-deficient

    Promoter

    TRE

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dox-Akt

    Plasmid

    #207328

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    rac protein kinase-alpha (AKT1 Human)
    

    Use

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 129 - 480

    Promoter

    Tet Response Element

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPRIME-TREX-GFP-FF3

    Plasmid

    #11667

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA-sfGFP

    Plasmid

    #162689

    Purpose

    pFA vector control expressing sfGFP

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    sfGFP
    

    Use

    Tags

    C terminal 6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    PL tet promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-TODestFLAGHA_RBPMS2_HindIII

    Plasmid

    #65666

    Purpose

    FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVUT-spC3GFP

    Plasmid

    #225487

    Purpose

    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Secretable and permeable exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TODestFLAGHA_RBPMS_KDR

    Plasmid

    #65091

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed sequence to render plasmid resistant to s…

    Promoter

    CMV, 2x Tet

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT-TODestMYC_RBPMS_KDR

    Plasmid

    #65667

    Purpose

    MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    introduced HindIII site to exchange FLAG-HA tag w…

    Promoter

    CMV, 2x Tet

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFRT-TODestFLAGHA_RBPMS_KDR_K100E

    Plasmid

    #65093

    Purpose

    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 100 to glutamic acid (KDR backgrou…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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