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pYPQAT04

Plasmid

#223376

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.2-Dadh-ΔElen0470(ElA2)

Plasmid

#225317

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0470) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0470)

Tags

His8

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Tags

HisTag

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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YX022 (PB)EF1a-LIDARsensor (hygR)

Plasmid

#233431

Purpose

EF1a-driven LIDAR reporter RNA

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN1-C1858_02810-(–)-Eldh2-noAccs (ElA2)

Plasmid

#225309

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh2 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3

Plasmid

#233421

Purpose

IVT template for CCR6-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-CCR6-V2-ADAR2dd-hybridUTR3

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ076 (PB) CMV-mCCL20 (PuroR-BFP)

Plasmid

#233433

Purpose

PiggyBac donor plasmid, membrane-bound CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ033 CMV-TO-CCL20-pre-mGRASP

Plasmid

#233417

Purpose

plasmid expressing membrane-bound CCL20 by fusing with pre-mGRASP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN)

Plasmid

#233437

Purpose

SFFV driven AVPR2-ADAR2dd (as in XZ030)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM

Plasmid

#233428

Purpose

LIDAR reporter RNA with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-sensor(tight)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)

Plasmid

#233438

Purpose

SFFV driven CCR6-ADAR2dd (as in XZ032)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ073 (IVT) CMV-TO-T7-LIDARsensor-hybridUTR3

Plasmid

#233420

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, EGFP as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-LIDARsensor-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ089 (IVT) CMV-TO-T7-ARRB2-MCP-hybridUTR3

Plasmid

#233422

Purpose

IVT template for ARRB2-tdMCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-ARRB2-MCP-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ387 (IVT) CMV-TO-T7-AVPR2-V2-ADAR2dd-hybridUTR3

Plasmid

#233423

Purpose

IVT template for AVPR2-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-AVPR2-V2-ADAR2dd-hybridUTR3

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0040 Ac5-FKBP-ADARdd

Plasmid

#233411

Purpose

AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233402

Purpose

_-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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LSM060 CMV-TO-AVPR2-(10aa-linker)-MCP (FLP-IN)

Plasmid

#233403

Purpose

AVPR2-V2-tdMCP with a 10 amino acid linker and extra V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233391

Purpose

IGF1R-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IGF1R-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A)

Plasmid

#233393

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
XZ032 CMV-TO-CCR6-V2-ADAR2dd(E488Q, T501A)

Plasmid

#233394

Purpose

CCR6-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
YH038 PGK-TO-FRB-tm-MCP

Plasmid

#233377

Purpose

PGK promoter driven FRB fused with MCP, with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-CD28TM-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF1-C1858_02810-(–)-Eldh2-Accs (ElA2)

Plasmid

#225296

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh2 in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF5-Elen0515-eCadh-Accs(ElA2)

Plasmid

#225300

Purpose

Expresses Eggerthella lenta A2 eCadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

eCadh

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.3-Dadh-ΔElen0473(ElA2)

Plasmid

#225318

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0473) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0473)

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.6-Dadh-ΔElen0476(ElA2)

Plasmid

#225321

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0476) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0476)

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80EN2-Elen0501-(+)-Eddh-noAccs(ElA2)

Plasmid

#225310

Purpose

Expresses Eggerthella lenta A2 (+)-Eddh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80EN5-Elen0515-eCadh-noAccs(ElA2)

Plasmid

#225313

Purpose

Expresses Eggerthella lenta A2 eCadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

eCadh (Elen_0515 )

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

Plasmid

#225315

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0469)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

Plasmid

#225319

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0474)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge