We narrowed to 8,554 results for: sgRNA

1. pYPQAT37

   Plasmid

   #223409

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT40

   Plasmid

   #223412

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lsg-GBA-1 + 2

   Plasmid

   #199276

   Purpose

   nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. epiCRISPR

   Plasmid

   #135960

   Purpose

   Expresses SpCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR1319

   Plasmid

   #111116

   Purpose

   Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   UBE2T gRNA (UBE2T Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. RRM1 E1.3 gRNA

   Plasmid

   #90880

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM1 (Guide Designation E1.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJY-RpABE

   Plasmid

   #112872

   Purpose

   binary vector for ABE expression in Arabidopsis

   Depositor

   Jae-Young Yun, Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. RRM1 E2.3 gRNA

   Plasmid

   #90881

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM1 (Guide Designation E2.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RRM2 E4.3 gRNA

   Plasmid

   #90883

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RRM2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   RRM2 (Guide Designation E4.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RRM2 E3.3 gRNA

    Plasmid

    #90882

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS156

    Plasmid

    #182707

    Purpose

    CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

    Depositor

    Rebecca Shapiro

    Article

    Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPR-V2_hMSH2

    Plasmid

    #186155

    Purpose

    sgRNA targeting human MSH2 gene

    Depositor

    David McFadden

    Article

    Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

    Insert

    MSH2 (MSH2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-SAM-Dest

    Plasmid

    #102563

    Purpose

    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PL6-CS-WR

    Plasmid

    #122512

    Purpose

    construction for sgRNA transcription with Plasmodium falciparum U6 promotor

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PfU6

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. BRCA2 A12.2 gRNA

    Plasmid

    #90551

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SiC-V1

    Plasmid

    #133041

    Purpose

    SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

    Plasmid

    #187925

    Purpose

    sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

    Depositor

    Martin Turner

    Article

    Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

    Insert

    hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

    Plasmid

    #169449

    Purpose

    expression of TRF1-mcherrry and sgRNA targeting telomeres

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    mCherry-TRF1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge