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pEGFPc1 PTEN C124S

Plasmid

#237340

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Publication

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXL2

Plasmid

#236455

Purpose

transient overexpression of FBXL2 in mammalian cells

Depositor

Michele Pagano

Publication

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

FBXL2 (FBXL2 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

Plasmid

#200845

Purpose

Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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GvpA-CaM

Plasmid

#223555

Purpose

Full length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.

Depositor

Jean Gariepy

Publication

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET300-GvpACaM

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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act-3p(long):rfp

Plasmid

#202330

Purpose

The act-3 promoter drives expression of TurboRFP in the pharynx, spermatheca, and body wall of C. elegans.

Depositor

Didier Stainier

Publication

Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

Inserts

act-3p(long) promoter
TurboRFP

Expression

Worm

Promoter

This is a promoter sequence for C. elegans act-3 …

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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eft-3p:act-5(ptc):tbb2 3' UTR

Plasmid

#202331

Purpose

The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

Depositor

Didier Stainier

Publication

Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

Inserts

eft-3 promoter
act-5(ptc)
tbb-2 3' UTR

Tags

base 238 C -> A mutation causing a PTC at codo…

Expression

Worm

Promoter

This is a promoter sequence for C. elegans eft-3,…

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12561

Plasmid

#219922

Purpose

PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter PGM

Expression

Yeast

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12565

Plasmid

#219925

Purpose

PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter FBA

Expression

Yeast

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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