We narrowed to 10,577 results for: Kars

1. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TT Tgfbr3-HA

   Plasmid

   #58497

   Purpose

   Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Tgfbr3_HA (Tgfbr3 Mouse)
   

   Use

   Entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGEX Aps-SH2

   Plasmid

   #46404

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Aps (SH2B2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 404-523

   Promoter

   Tac

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b-Strep-SsOGT-H5-His

   Plasmid

   #167277

   Purpose

   Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SsOGT-H5
   

   Use

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2xFKBP-myc

   Plasmid

   #20212

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFKBP (FKBP1A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. Myc-CD4-mCherry-HA-FRB

   Plasmid

   #100740

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD4
   

   Use

   Tags

   FRB, HA, Myc, and mCherry

   Expression

   Mammalian

   Mutation

   Engineered to remove large part of extracellular …

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5 Flag Jnk1a1(APY)

   Plasmid

   #15742

   Depositor

   Roger Davis

   Article

   Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

   Insert

   Jnk1a1(TPF) (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Thr-190Ala

   Promoter

   Available since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pTRIPZ shCUX1-5328

   Plasmid

   #100815

   Purpose

   Lentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099

   Depositor

   Alain Nepveu

   Article

   Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

   Insert

   TGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Vh-HA-2xFKBP

   Plasmid

   #20221

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFKBP (FKBP1A Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pSMP-MBD2_2

    Plasmid

    #36369

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD2 (MBD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only