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YIplac128-pOst1-ESCargo(Orange)

Plasmid

#140158

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo(Crimson)

Plasmid

#140156

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Publication

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1962

Plasmid

#82572

Purpose

High copy number plasmid containing Ple303 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple303

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1963

Plasmid

#82573

Purpose

High copy number plasmid containing Ple304 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple304

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1961

Plasmid

#82571

Purpose

High copy number plasmid containing Ple302 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple302

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1960

Plasmid

#82570

Purpose

High copy number plasmid containing Ple301 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple301

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1957

Plasmid

#82568

Purpose

High copy number plasmid containing hs1218 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

hshs1218

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1953

Plasmid

#82567

Purpose

High copy number plasmid containing hs671 MiniPromoter insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

hs671

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1944

Plasmid

#82566

Purpose

High copy plasmid containing Ple267 (UGT8 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple267

Use

Pleiades promoter project [sic, pleaides plieades]

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCRII-Sema4f-ISH-2kb

Plasmid

#68033

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4F (Sema4f Mouse)

Use

Mrna in situ hybridization probe

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBS ZfHoxb9a

Plasmid

#27529

Depositor

Victoria Prince

Publication

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxb9a (hoxb9a Zebrafish)

Use

Cloning vector

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSP64TNE Xnr4 (DM#174)

Plasmid

#15078

Depositor

Douglas Melton

Publication

Joseph et al Dev Biol. 1997 Apr 15. 184(2):367-72.

Insert

Xnr4 (xnr4 Frog)

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pCM61

Plasmid

#261133

Purpose

For N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.

Depositor

John Calarco

Publication

Martin et al bioRxiv 2025.02.28.640866

Insert

AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC

Expression

Worm

Promoter

none

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCM60

Plasmid

#261132

Purpose

For N-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.

Depositor

John Calarco

Publication

Martin et al bioRxiv 2025.02.28.640866

Insert

AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC

Expression

Worm

Promoter

none

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCM62

Plasmid

#261130

Purpose

For C-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.

Depositor

John Calarco

Publication

Martin et al bioRxiv 2025.02.28.640866

Insert

AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC

Expression

Worm

Promoter

none

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM-T_hmx3a

Plasmid

#254517

Purpose

Partial hmx3a cDNA. For in vitro transcription of ISH probe

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

hmx3a (hmx3 Zebrafish)

Use

Zebrafish expression

Promoter

SP6

Available since

Sept. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCM63

Plasmid

#261131

Purpose

For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.

Depositor

John Calarco

Publication

Martin et al bioRxiv 2025.02.28.640866

Insert

AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC

Expression

Worm

Promoter

none

Available since

Aug. 31, 2026

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G_Gss-NodalF1-Myc-LaG16-LaG2_PGK_BFP

Plasmid

#259149

Purpose

Inducible expression of a secreted anti-GFP nanobody dimer fused to the Finger 1 domain of the mouse Nodal1 protein.

Depositor

Leonardo Morsut

Publication

Swedlund et al bioRxiv [Preprint]. 2026 May 25:2026.05.

Insert

anti-GFP-NodalF1

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-3G

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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