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ADEPT-pTarget-tetR

Plasmid

#238043

Purpose

Modified ADEPT-pCas9 with sfGFP under TtrB promoter for tetrathionate (TTR) sensing.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

ttrB

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-PKD

Plasmid

#237521

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_pseudokinase

Expression

Bacterial

Mutation

Pseudokinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca+C-Tail

Plasmid

#237526

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Yeast

Mutation

Add C-terminal extension

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a:His-MBP-KD

Plasmid

#237520

Purpose

Recombinant protein expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4_kinase

Expression

Bacterial

Mutation

Kinase region of RWT4

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca_Reg1

Plasmid

#237512

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Plant

Mutation

Removed binding region 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19::ZmUBI-RWT4Ca+C-Tail

Plasmid

#237514

Purpose

Protoplast expression

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Plant

Mutation

Add C-terminal extension

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

Plasmid

#236253

Purpose

Dre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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YX022 (PB)EF1a-LIDARsensor (hygR)

Plasmid

#233431

Purpose

EF1a-driven LIDAR reporter RNA

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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XZ088 (IVT) CMV-TO-T7-CCR6-V2-ADAR2dd-hybridUTR3

Plasmid

#233421

Purpose

IVT template for CCR6-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-CCR6-V2-ADAR2dd-hybridUTR3

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ076 (PB) CMV-mCCL20 (PuroR-BFP)

Plasmid

#233433

Purpose

PiggyBac donor plasmid, membrane-bound CCL20

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ033 CMV-TO-CCL20-pre-mGRASP

Plasmid

#233417

Purpose

plasmid expressing membrane-bound CCL20 by fusing with pre-mGRASP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0031 SFFV-AVPR2-ADAR2dd (FLP-IN)

Plasmid

#233437

Purpose

SFFV driven AVPR2-ADAR2dd (as in XZ030)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR2-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ087 SFFV-mCherry-sensor(tight)-hIgG1-TM

Plasmid

#233428

Purpose

LIDAR reporter RNA with a membrane-bound human IgG as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-sensor(tight)-hIgG1-TM

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0043 SFFV-CCR6-ADAR2dd (FLP-IN)

Plasmid

#233438

Purpose

SFFV driven CCR6-ADAR2dd (as in XZ032)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

SFFV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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APL0040 Ac5-FKBP-ADARdd

Plasmid

#233411

Purpose

AC5 promoter driven FRBP-ADAR2dd for drosophila cell expression

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

Ac5

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ038 CMV-TO-IGF1R-ADAR2(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233391

Purpose

IGF1R-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IGF1R-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ031 CMV-TO-CCR6-ADAR2dd(E488Q, T501A)

Plasmid

#233393

Purpose

CCR6-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and no V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ032 CMV-TO-CCR6-V2-ADAR2dd(E488Q, T501A)

Plasmid

#233394

Purpose

CCR6-V2-ADAR2dd(E488Q, T501A) with a short 4 amino acid linker and V2 tail

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ110 CMV-TO-signal-B2AR-V2-ADAR2dd(DD-E488Q-T501A) (FLP-IN)

Plasmid

#233402

Purpose

_-2 adrenergic receptor-V2-ADAR2dd(E488Q, T501A)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

B2AR-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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YH038 PGK-TO-FRB-tm-MCP

Plasmid

#233377

Purpose

PGK promoter driven FRB fused with MCP, with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-CD28TM-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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