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pnEA-NpM-MCS1-GB1_AB

Plasmid

#148370

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-MCS2-GB1_AB

Plasmid

#148371

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pM-mRuby2

Plasmid

#176550

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_mcherry

Plasmid

#167870

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pL-mWasabi

Plasmid

#176552

Purpose

Expression of mWasabi for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

mWasabi

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pM-eCFP

Plasmid

#176558

Purpose

Expression of eCFP for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pH-TagRFP657

Plasmid

#176560

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of lysine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_iRFP713

Plasmid

#167868

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Hygro

Plasmid

#167867

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EBFP2

Plasmid

#167865

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP1H5

Plasmid

#165065

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_STS:P4S

Plasmid

#139797

Purpose

expression of biosynthetic enzyme STS in fusion with CC segment P4S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS:P4S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pStA0::crtB

Plasmid

#114344

Purpose

crtB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtB (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::dxs

Plasmid

#114346

Purpose

dxs in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

dxs (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtZ

Plasmid

#114348

Purpose

crtZ in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtZ (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::lcyB

Plasmid

#114349

Purpose

lcyB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

lcyB (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::idi

Plasmid

#114350

Purpose

idi in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

idi (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::J23118

Plasmid

#114332

Purpose

Promoter J23118 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23118

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc13

Plasmid

#114333

Purpose

RBS RBSc13 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc13

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc33

Plasmid

#114334

Purpose

RBS RBSc33 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc33

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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