We narrowed to 59,355 results for: cloning

1. pQTEV-RPL9

   Plasmid

   #31334

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   ribosomal protein L9 (RPL9 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK019

   Plasmid

   #105378

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor EXP7 (At1g12560; 750bp)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSKB-

   Plasmid

   #62540

   Purpose

   Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

   Plasmid

   #232127

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-PPP1R1A

   Plasmid

   #31331

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   protein phosphatase 1 (PPP1R1AP1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQTEV-CIP29

   Plasmid

   #34822

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   cytokine induced protein 29 kDa (SARNP Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO HSPA1L

    Plasmid

    #19457

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1L (HSPA1L Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5238

    Plasmid

    #128711

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopG1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEG R2-L3 #BGV193

    Plasmid

    #48952

    Purpose

    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pENTR-TER+-shLKB1-Hu

    Plasmid

    #61243

    Purpose

    Entry clone encoding shRNA to human LKB1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    LKB1 (STK11 Human)
    

    Use

    Entr clone

    Promoter

    H1/TO

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5/FRT/TO GFP DNAJB8

    Plasmid

    #19504

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB8 (DNAJB8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+/N-Halo

    Plasmid

    #184417

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQTEV-SNX3

    Plasmid

    #31573

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sorting nexin 3 (SNX3 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-CR7T-gRNA2.1-nlsBFP

    Plasmid

    #170515

    Purpose

    gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    CR7T

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV U6-sgRNA UbC-tdTomato

    Plasmid

    #106949

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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