Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 62,913 results for: cloning

Clear keywords
Showing: 6601 - 6620 of 62913 results

  1. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

    Enlarge

  2. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid
    #129444
    Purpose
    expression of FnCas9 and DsRed
    Depositor
    Oskar Laur
    Insert
    FnCas9 IRES-Dsred
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129444-plasmid-map-sequence-id-256492

    Enlarge

  3. pFH102

    Plasmid
    #128207
    Purpose
    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Publication
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128207-plasmid-map-sequence-id-252909

    Enlarge

  16. pCWX-R4DESTR2-PGK-BSD

    Plasmid
    #114320
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistance
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114320-plasmid-map-sequence-id-219986

    Enlarge

  17. mEos4b-CenpA-C-22

    Plasmid
    #62775
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    CenpA (CENPA Human)
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62775-plasmid-map-sequence-id-173170

    Enlarge

  18. pHBJT034

    Plasmid
    #225180
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Publication
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225180-plasmid-map-sequence-id-444744

    Enlarge

  19. AD-003

    Plasmid
    #25502
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AD-003 (NTMT1 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. FXR2

    Plasmid
    #25327
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FXR2_19 (FXR2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6601 - 6620 of 62913 results