We narrowed to 8,554 results for: sgRNA

1. MAD2L1BP F2.1 gRNA

   Plasmid

   #90745

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MAD2L1BP (Guide Designation F2.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL11058

   Plasmid

   #68254

   Purpose

   Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter_TaU6 + sgRNA_HvPM19_3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-U6-CNCB

   Plasmid

   #236763

   Purpose

   A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter, CAG promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-2G-CNCB

   Plasmid

   #236764

   Purpose

   A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter, human U6 promoter, CAG promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPH07

   Plasmid

   #234345

   Purpose

   Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

   Depositor

   Jerome Fox

   Article

   Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

   Insert

   Type II dCas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT33

   Plasmid

   #223405

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT34

   Plasmid

   #223406

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT35

   Plasmid

   #223407

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT36

   Plasmid

   #223408

   Purpose

   T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpCas9-D10A-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEIF3D-WT-sgNT

    Plasmid

    #222120

    Purpose

    EIF3D overexpression vector with non-targeting sgRNA

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGM65

    Plasmid

    #220170

    Purpose

    CRISRPi guide RNA against NACA

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA (sgRNA) (NACA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPRRv2_sgTom

    Plasmid

    #218346

    Purpose

    CRISPR KO plasmid for tdTomato in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting tdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGM133

    Plasmid

    #220173

    Purpose

    CRISRPi guide RNA against TTC1

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TTC1 (sgRNA) (TTC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2ss-U6-sgGFP

    Plasmid

    #190899

    Purpose

    AAV vector expressing sgGFP

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

    Plasmid

    #192131

    Purpose

    Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDe_Cas9_KANA_R160a

    Plasmid

    #173756

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160a_sgRNA2/R160a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDe_Cas9_KANA_R160b

    Plasmid

    #173757

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/R160b_sgRNA2/160b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDe_Cas9_KANA_R390a

    Plasmid

    #173758

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

    Depositor

    Kristina Gruden

    Article

    Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

    Insert

    Cas9_sgRNA1/390a_sgRNA2/390a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6 &StU6

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dora[B]_rescue_construct

    Plasmid

    #190637

    Purpose

    Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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