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We narrowed to 14,290 results for: flag

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Showing: 6641 - 6660 of 14290 results

  1. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

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  5. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169184
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169184-plasmid-map-sequence-id-333981

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  7. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  8. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid
    #50896
    Purpose
    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L717C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50896-plasmid-map-sequence-id-76369

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  15. pCS2+8-CYP2A6(C14A, C439A)-flag-tev-halo-his

    Plasmid
    #227162
    Purpose
    For T-REX experiments of CYP2A6 C14A and C439A double mutant. Less sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 14 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227162-plasmid-map-sequence-id-459059

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  16. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

    Plasmid
    #227163
    Purpose
    For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 82 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227163-plasmid-map-sequence-id-459000

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  19. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240513-plasmid-map-sequence-id-477190

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Showing: 6641 - 6660 of 14290 results