159,371 results

1. pNCS BFP

   Plasmid

   #91757

   Purpose

   Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Blue Fluorescent Protein
   

   Use

   Tags

   Hexa-Histidine, Xpress epitope for detection with…

   Expression

   Bacterial

   Mutation

   GFP (Y66H)

   Promoter

   T7

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST53-LRRK2-WT

   Plasmid

   #25044

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

   Plasmid

   #40640

   Depositor

   Brenda Lilly

   Article

   Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

   Insert

   NICD3 (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide_DKO

   Plasmid

   #183193

   Purpose

   Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, mU6

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/ATP10D-HA

   Plasmid

   #209217

   Purpose

   Mammalian expression of ATP10D

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP10D (ATP10D Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSFFV_mNG3A(1-10)

   Plasmid

   #157992

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   pSFFV_mNG3K(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   W148L, M150V, K153M, N194K, F204L

   Promoter

   SFFV

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-EIN-FTO(32-505)

   Plasmid

   #186466

   Purpose

   Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   Fat mass obesity associated protein (FTO Human)
   

   Use

   Tags

   6 Histidine-EIN (N-terminal domain of E.coli Enzy…

   Expression

   Bacterial

   Mutation

   amino acid 32-505

   Promoter

   T7

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBITE-CD19-CD3

   Plasmid

   #190677

   Purpose

   Encodes a modularized form of HD37-OKT3 bispecific antibody

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Modularized-HD37-modularized-OKT3-P2A-NeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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