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pCB092 FoMV-sgMgCh

Plasmid

#234815

Purpose

To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

Depositor

Daniel Voytas

Publication

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting SbMgCh

Expression

Plant

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcLF1

Plasmid

#198581

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Publication

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcLF1

Use

Gateway Cloning

Mutation

None (wildtype)

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD3

Plasmid

#195841

Purpose

Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

Depositor

Gunnar Schulte

Publication

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD3 (FZD3 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

I292V and V495I

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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(401) pAAV CMV-ZsGreen U6-gRNA

Plasmid

#163021

Purpose

Fluorescent reporter for Sa gRNA (Bsa1 sites)

Depositor

Tonia Rex

Publication

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Zs Green

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry 14-3-3β

Plasmid

#111880

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

Depositor

Jesse Rinehart

Publication

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: 14-3-3β fused to C-terminal…

Expression

Bacterial

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Publication

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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HA-POMP

Plasmid

#86765

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human POMP (POMP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-HA-TRAF4

Plasmid

#58314

Purpose

HA-tagged mouse TRAF4

Depositor

Ying Zhang

Publication

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_SRF

Plasmid

#72695

Purpose

Doxycyline regulated expression of SRF in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

serum response factor (SRF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMWCAVI_H6BAP-OROV_N

Plasmid

#240162

Purpose

Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

Depositor

Stephen Graham

Publication

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

Expression

Bacterial

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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PB OROV Gc Spike H6

Plasmid

#229662

Purpose

Make PiggyBac stable cell line expressing Oropouche virus BeAn 19991 Gc spike region (residues 482–894 of M polyprotein) with C-terminal His6 tag

Depositor

Stephen Graham

Publication

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

OROV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 482-894 only

Promoter

TRE

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5 n-myc mPirh2

Plasmid

#212994

Purpose

expresses mouse PIRH2

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

pirh2 (Rchy1 Mouse)

Tags

MYC

Expression

Mammalian

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-HA AXIN1 delta PHGDH (delta 321-340)

Plasmid

#213005

Purpose

expresses mouse AXIN1

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

AXIN1 (Axin1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-FLAG HIPK2

Plasmid

#213004

Purpose

expresses mouse HIPK2

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

HIPK2 (Hipk2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH T57A

Plasmid

#212996

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

T57A

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI TP53 S46A

Plasmid

#213001

Purpose

expresses human P53

Depositor

Sheng-cai Lin

Publication

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

P53 (TP53 Human)

Expression

Mammalian

Mutation

S46A

Promoter

cmv

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-rS2d

Plasmid

#183516

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C

Depositor

Priyamvada Acharya

Publication

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

rS2d (S Severe acute respiratory syndrome coronavirus 2)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgCHMP1A

Plasmid

#169321

Purpose

Expresses CHMP1a targeting sgRNA in non-lentiviral BPK1520 plasmid

Depositor

Francisca Vazquez

Publication

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

sgCHMP1A

Use

CRISPR

Expression

Mammalian

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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BPK1520 sgCHMP4B

Plasmid

#169322

Purpose

Expresses CHMP4B targeting sgRNA in non-lentiviral BPK1520 plasmid

Depositor

Francisca Vazquez

Publication

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

sgCHMP4B

Use

CRISPR

Expression

Mammalian

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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