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pscAAV- EF1α core -hKCNJ2-V77E-HA tag

Plasmid

#246996

Purpose

Can be used to generate AAV virus that will express human KCNJ2 V77E mutant with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

KCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Mutation

V77E

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT8-neo

Plasmid

#250316

Purpose

Encodes gRNA targeting FUT8

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT8 gRNA (FUT8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mDE-Myc-His

Plasmid

#249180

Purpose

AAV mediated overexpression of mouse Sparcl1 a.a. 330-550 fragment

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAP-Tag5-IgKsp-DE-Myc-His

Plasmid

#249176

Purpose

Overexpression of mouse Sparcl1 fragment a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pAvA143

Plasmid

#248147

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen3 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiX1-CAG-mRuby2-HRAS(G12V)

Plasmid

#244165

Purpose

Lentiviral expression of mRuby2-HRAS(G12V)

Depositor

Kate Galloway

Article

Lende-Dorn et al Cell Rep. 2025 Sep 23;44(9):116226. doi:

Insert

mRuby2-HRAS(G12V) (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK ΔCRD-Fc

Plasmid

#246706

Purpose

Expression vector for secreted human RECK lacking the CRD, fused to mouse IgG2a Fc

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

Δaa 343-476, ΔGPI anchor signal

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK ΔCK-Fc

Plasmid

#246705

Purpose

Expression vector for secreted human RECK lacking CC domains 1-5, fused to mouse IgG2a Fc

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

Δaa 37-338, ΔGPI anchor signal

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHBT-NRAS174

Plasmid

#248051

Purpose

To express His6 tag-Avi tag-TEV cleavage site-NRAS (residues 1-174) in E. coli, under the T7 promoter.

Depositor

Shohei Koide

Article

Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

NRAS (NRAS Human)

Tags

His6 tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

includes aa 1-174 only

Promoter

T7

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-FGF6-V5

Plasmid

#124090

Purpose

FGF6-V5 tagged expression vector

Depositor

J. Chad Brenner

Article

Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

Insert

FGF6 (FGF6 Human)

Use

Lentiviral

Tags

V5

Mutation

3 silent SNPs: (189) C>G, (366) T>C and (41…

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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Flag- Src H492A

Plasmid

#246425

Purpose

Expresses Src mutant H492A (aka H495A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Histidine 492 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src H201A

Plasmid

#246424

Purpose

Expresses Src mutant H201A (aka H204A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed His 204 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 E252A

Plasmid

#244491

Purpose

Bacterial Expression of SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Glutamate 252 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src E524A

Plasmid

#246426

Purpose

Expresses Src mutant E524A (aka E527A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Glu 527 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A

Plasmid

#246423

Purpose

Expresses Src mutant C185A or C188A in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Cys 188 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NES-CAGEc-ETOc (Npu)

Plasmid

#241409

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NES-CAGEc-ETOc (Npu) (RUNX1T1 Synthetic, Human)

Tags

3xFLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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NPM1c-TurboID-MSCV-IRES-GFP

Plasmid

#240129

Purpose

Expresses mutant NPM1c-TurboID fusion in mammalian cells

Depositor

Timothy Ley

Article

Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

Insert

NPM1c (NPM1 Human)

Use

Retroviral

Tags

IRES-GFP and TurboID

Expression

Mammalian

Mutation

C-terminal frameshift mutation as seen in Acute M…

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2294

Plasmid

#228352

Purpose

Methanol-inducible ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Promoter

KpAOX1 promoter

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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