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POT7-RFP

Plasmid

#65323

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT6-RFP

Plasmid

#65322

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Publication

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pScPPX1

Plasmid

#233713

Purpose

Bacterial expression of the yeast PPX1 gene

Depositor

Michael Downey

Publication

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

ScPpx1 (PPX1 Budding Yeast)

Expression

Bacterial

Available since

May 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPEY-Z3-Nat

Plasmid

#232100

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo-AID*-GFP

Plasmid

#212775

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-NUbo-GFP-His6

Plasmid

#212790

Purpose

Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-GFP-His6

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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EMC1-2EGFP-ter-NAT

Plasmid

#184763

Purpose

Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

EMC1 (EMC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Tags

EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ABF2-2EGFP-ter-URA3

Plasmid

#184768

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ABF2 (ABF2 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTPI1-EMC1-GFP-APEX2-URA3

Plasmid

#184776

Purpose

Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

APEX2

Expression

Yeast

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 WT

Plasmid

#169261

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

Depositor

Susan Baserga

Publication

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 WT (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Publication

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Expression

Yeast

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-Elo3-GFP11-Ura

Plasmid

#177734

Purpose

ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

Depositor

Erik Böttger

Publication

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

Elo3 (ELO3 Budding Yeast)

Tags

GFP11 and HDEL

Expression

Bacterial and Yeast

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Publication

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Expression

Yeast

Promoter

VTC4 (native promoter)

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAG415-ADH1p-HA-rap1-K9R

Plasmid

#84531

Purpose

This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

HA

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

ADH1

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Publication

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only