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pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

Plasmid

#213535

Purpose

Expresses the split transcription factors in cultured neuron and mouse brain

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

Synapsin

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp KI;Empty

Plasmid

#240302

Purpose

Empty vector

Depositor

Yuki Ogawa

Insert

Empty gRNA

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-Flex-MCS-WPRE-SV40 - GCH26

Plasmid

#230827

Purpose

AAV cloning vector for CRE-dependent expression under the TRE promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV and Cre/Lox; Cloning vector

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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CaMKII-Flex-MCS-WPRE-SV40 - GCH20

Plasmid

#230824

Purpose

AAV cloning vector for CRE-dependent expression under the CaMKII promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV and Cre/Lox; Cloning vector

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-Bi-DREADD

Plasmid

#159457

Purpose

AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

Bi-DREADD

Expression

Mammalian

Mutation

fusion protein of hM3Dq-mCherry and HA-KORD seper…

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pC13P-iCLHN

Plasmid

#66580

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Nanoluc-HaloTag

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp Donor;smFP-V5 KO;Template ORF0

Plasmid

#240304

Purpose

Donor:smFP-V5 KO:Template

Depositor

Yuki Ogawa

Insert

Template gRNA

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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AiP11763: pAAV-hEF1a-DIO-ChR2(CRC)-EYFP-WPRE-HGHpA (Alias: CN1763)

Plasmid

#236602

Purpose

A vector for Cre dependent expression of ChR2(CRC)-EYFP; CRC variant contains mutations L132C, H134R, and T159C in ChR2

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

ChR2(CRC)-EYFP

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGolden-Hyg

Plasmid

#51423

Purpose

Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK-Hyg-pA (PGK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAd5Rep2entire-ATGf

Plasmid

#237878

Purpose

AAV packaging plasmid: This platform plasmid provides adenoviral helper functions and the AAV2 rep gene.

Depositor

Ascend Advanced Therapies, Florian Sonntag

Insert

rep2 and E2A, E4, L4-22K/33K, VA RNA

Use

AAV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9_sgAAVS1

Plasmid

#199213

Purpose

All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

AAVS1-gRNA

Use

CRISPR

Promoter

human U6

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-FRT-MCS-WPRE-SV40 - GCH27

Plasmid

#230828

Purpose

AAV cloning vector for Flpo-dependent expression under the TRE promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector, flp/frt

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1994

Plasmid

#49124

Purpose

AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple266-iCre

Use

AAV

Expression

Mammalian

Promoter

S100B

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTMB304_ZFcharm_Kv1_Prnp_SCR

Plasmid

#220842

Purpose

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv1

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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