We narrowed to 53,200 results for: Ran

1. pTH862-CEN-slowmaxRLuc/minCFLuc

   Plasmid

   #115371

   Purpose

   Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

   Depositor

   Tobias von der Haar

   Inserts

   Firefly Luciferase (codon minimised)
   Gcn4 5'-UTR + Renilla luciferase (codon optimised)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons have been changed for the most favoura…

   Promoter

   ADH1 and TDH3

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-4T-3-cytXlATL2

   Plasmid

   #124063

   Purpose

   Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXIATL2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYES260-sc.eEF2 K509A

   Plasmid

   #85120

   Purpose

   yeast expression of mutant EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K509A

   Promoter

   GAL1

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P-1 BICC FL

   Plasmid

   #112998

   Purpose

   For protein expression and purification of full-length Drosophila BicC

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length BicC (BicC Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a - Ec-ClpX D144L

   Plasmid

   #111514

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Aspartatic acid 144 to Leucine

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AY23_pgRNA.R5.2

   Plasmid

   #100295

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-RP2-C86Y,P95L

   Plasmid

   #86074

   Purpose

   A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   RP2 (RP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed cysteine-86 and proline-95 to tyrosine an…

   Promoter

   tac

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-cystin-CTS

   Plasmid

   #86066

   Purpose

   A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Cystin (Cys1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 28-35

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

    Plasmid

    #61796

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

    Plasmid

    #61793

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

    Plasmid

    #61791

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

    Plasmid

    #61790

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

    Plasmid

    #61789

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    both miR-200c MRE-2 and MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

    Plasmid

    #61786

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Crtap 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pLexA::SRDX (GB0732)

    Plasmid

    #68184

    Purpose

    Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    LexA Binding Domain fused to SRDX Transcriptional Repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOSGE_COF3H09

    Plasmid

    #71003

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    SNF4Agamma (SNF4Agamma Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pFOSGE_COF3C07

    Plasmid

    #70985

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    14-3-3zeta (14-3-3zeta Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pFOSGE_COF2B09

    Plasmid

    #70918

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    gro (gro Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFOSGE_COF3C03

    Plasmid

    #70959

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf4 (Taf4 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only