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Plasmid#117836PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coliDepositorInsertpromoter, RBS, meffRed
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST-pyrE
Plasmid#203813PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB521
Plasmid#74863PurposeGateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK005.6
Plasmid#153025PurposedCas9, MCP-SoxS(R93A/S101A), J106 scRNADepositorInsertdCas9, MCP-SoxS(R93A/S101A), J106 scRNA
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB420
Plasmid#74814PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB450
Plasmid#74832PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB410
Plasmid#74804PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXNPH3
Plasmid#110098PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE670
Plasmid#153236PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein promoter-less
Plasmid#118006PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilGFP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB520
Plasmid#74862PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
UseTagsExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR358.4
Plasmid#125114PurposeExpression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121DepositorInsertsnarL(REC)-liaR(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectMutationPromoterDrosophila Synthetic Core PromoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB418
Plasmid#74812PurposeGateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB508
Plasmid#74850PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
tsPurple chromoprotein promoter-less
Plasmid#118007PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, tsPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.10
Plasmid#125106PurposeExpression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only