We narrowed to 10,494 results for: nar

1. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcBac1.tR4-AcdRS82

   Plasmid

   #174101

   Purpose

   Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   M. barkeri tRNA synthetase for acridone82
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Intersectin Short

   Plasmid

   #47394

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Intersectin Long

   Plasmid

   #47395

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-dLight1.2

   Plasmid

   #111068

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1 and AAV5

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-MTOR-I2500F

   Plasmid

   #69014

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-I2500F (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Isoleucine 2500 to Phenylalanine

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

   Plasmid

   #35510

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

   Plasmid

   #63059

   Purpose

   Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   PKIalpha (Pkia Synthetic, Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJS647

   Plasmid

   #183176

   Purpose

   PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

   Depositor

   Timothy Whitehead

   Article

   Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

   Insert

   PYR1 (PYR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/TO-Strep/HA-UBE2J1

    Plasmid

    #124669

    Purpose

    Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRC/CMV-JAK1-VSV

    Plasmid

    #139356

    Purpose

    Expression of JAK1 protein

    Depositor

    Sandra Pellegrini

    Article

    Richter et al J Biol Chem. 1998 Sep 18;273(38):24723-9

    Insert

    JAK1 cDNA with 300nt of 5'UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGfa2-nLac

    Plasmid

    #53126

    Purpose

    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

    Depositor

    Michael Brenner

    Article

    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains -2163 to +47 (the human gfa2 segment), w…

    Promoter

    human GFAP

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. HaloTag-WDR5

    Plasmid

    #200880

    Purpose

    Mammalian expression of human WDR5 with N-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HaloTag-WDR5 (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid

    #184379

    Purpose

    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Tet3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

    Plasmid

    #100846

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. DPYD-FLAG

    Plasmid

    #61616

    Purpose

    The human DPYD was used to study the effect of DPYD expression on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (DPYD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. plentiCRISPv2_AAVS1

    Plasmid

    #184487

    Purpose

    Lentivirus all in one CRISPR vector targeting human AAVS1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    AAVS1 (AAVS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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