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Ad:2CMV_A2R2-N25K3

Plasmid

#122242

Purpose

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer20 CARD8-HA PAM

Plasmid

#169982

Purpose

Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

PAM

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

Plasmid

#108795

Purpose

Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment N-terminus to aa571

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

Plasmid

#108796

Purpose

Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa572 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#108769

Purpose

Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

Plasmid

#108737

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-entry+NLS

Plasmid

#128013

Purpose

Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4 DBD-SV40NLS-3x-Flag

Use

Gal4 entry vector with nls to generate tethering …

Tags

Gal4-DBD-SV40NLS-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

Plasmid

#121817

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway Cloning

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-B41gp160

Plasmid

#123247

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-001428gp160

Plasmid

#123255

Purpose

Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (001428) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111gp160

Plasmid

#123268

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q769gp160

Plasmid

#100928

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Promoter

None

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 S245X

Plasmid

#92366

Purpose

expression of M1 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso2 S172A

Plasmid

#74392

Purpose

Flag tagged human S172A MFF isoform 2 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-calx1

Plasmid

#58697

Purpose

Expression of calx1

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx1 (Calx Fly)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only