Addgene

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pRS414 Hsh155 K410N

Plasmid

#87280

Purpose

Encodes Hsh155 K410N for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

K410N

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSNAPf-AP

Plasmid

#61830

Purpose

Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

SNAP-AP

Use

Pcr template

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 USF2

Plasmid

#67069

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

USF2 (USF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TNF

Plasmid

#67062

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TNF (TNF Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 VPS29

Plasmid

#67052

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

VPS29 (VPS29 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 NFX1

Plasmid

#67131

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

NFX1 (NFX1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUC19 PKC zeta

Plasmid

#8425

Depositor

Frederic Mushinski

Article

Goodnight et al Gene 1992 Dec 15;122(2):305-11.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pJEP216-Lenti-1.3CaMKIIa P-GFP-WPRE-pA

Plasmid

#62926

Purpose

pLenti vector backbone designed to express GFP from a 1.3CaMKIIa promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pDC

Plasmid

#166941

Purpose

Dual control based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Dual Control

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pF-01

Plasmid

#166931

Purpose

GFP coding region part (F)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Green Fluorescent Protein - Block F- Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06

Plasmid

#67140

Purpose

Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 S100A1

Plasmid

#67048

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

S100A1 (S100A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSETT4tip

Plasmid

#153414

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Article

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

tipA promoter from Streptomyces lividans

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G09

Plasmid

#67143

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 SREBF2

Plasmid

#67129

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SREBF2 (SREBF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
env8 selectivity (d')

Plasmid

#99838

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (d')

Use

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 YY1

Plasmid

#67082

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YY1 (YY1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only