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Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only
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pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPiggyBacATCC LacZ GCTT loxAttP
Plasmid#173496PurposepiggyBac insect transgenesis destination vector for Golden Gate Cloning assembliesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA55
Plasmid#174768PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E without STOP codon
UseUnspecifiedTagsExpressionMutationL17EPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA56
Plasmid#174769PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E/D481V without STOP codon
UseUnspecifiedTagsExpressionMutationL17E/D481VPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA54
Plasmid#174767PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-D481V without STOP codon
UseUnspecifiedTagsExpressionMutationD481VPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA53
Plasmid#174766PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn without STOP codon
UseUnspecifiedTagsExpressionMutationWTPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
UseTagsExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZA52
Plasmid#174765PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E/D481V with STOP codon
UseUnspecifiedTagsExpressionMutationL17E/D481V + STOP codonPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA50
Plasmid#174763PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-D481V with STOP codon
UseUnspecifiedTagsExpressionMutationD481V + STOP codonPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA51
Plasmid#174764PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E with STOP codon
UseUnspecifiedTagsExpressionMutationL17E + STOP codonPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA49
Plasmid#174762PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
UseUnspecifiedTagsExpressionMutationWT + STOP codonPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0244
Plasmid#162282PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0024
Plasmid#162281PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY133
Plasmid#130961PurposeEngineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY131
Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only