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pEN_TmiR_Gb2-K

Plasmid

#25764

Purpose

Entry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD1

Plasmid

#210264

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD1

Promoter

CMV/TetO2

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 D394R

Plasmid

#210267

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 wt

Plasmid

#210266

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

mCherry

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD2

Plasmid

#210263

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD2

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 wt

Plasmid

#210262

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 wt siRES (NFATC2IP Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

siRES

Promoter

CMV/TetO2

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-K283)-FRB

Plasmid

#154451

Purpose

expresses flag-pBirA(1-K283)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-K283)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBACH2.2.0-gDNA

Plasmid

#176375

Purpose

Expresses spCas9 and gRNA sequence targeting BACH2

Depositor

Barbara Stranger, Kevin White

Insert

BACH2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSOX2.1.0-gDNA

Plasmid

#176382

Purpose

Expresses spCas9 and gRNA sequence targeting SOX2

Depositor

Barbara Stranger, Kevin White

Insert

SOX2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSOX11.1.0-gDNA

Plasmid

#176378

Purpose

Expresses spCas9 and gRNA sequence targeting SOX11

Depositor

Barbara Stranger, Kevin White

Insert

SOX11 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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3C8X

Plasmid

#51336

Depositor

Cheryl Arrowsmith

Insert

3C8X

Use

Baculovirus expression

Tags

His

Expression

Mutation

Truncation: See Comments

Promoter

T7

Available since

Feb. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pZBED9.1.1-gDNA

Plasmid

#175854

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

ZBED9-Nickase1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pZBED9.1.2-gDNA

Plasmid

#175853

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

ZBED9-Nickase2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDX4.1.1-gDNA

Plasmid

#175852

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CDX4-Nickase1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDX4.1.2-gDNA

Plasmid

#175851

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CDX4-Nickase2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTRERF1.1.0-gDNA

Plasmid

#176384

Purpose

Expresses spCas9 and gRNA sequence targeting TRERF1

Depositor

Barbara Stranger, Kevin White

Insert

TRERF1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pZNF627.1.0-gDNA

Plasmid

#176383

Purpose

Expresses spCas9 and gRNA sequence targeting ZNF627

Depositor

Barbara Stranger, Kevin White

Insert

ZNF627 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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