We narrowed to 14,439 results for: NTS

1. pGL4_CRE-CMVmin-luc2

   Plasmid

   #194384

   Purpose

   Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   6xCRE-CMVmin-luc2
   

   Expression

   Mammalian

   Promoter

   CMV minimal

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYL156 (TRV RNA2)

   Plasmid

   #148969

   Purpose

   Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

   Insert

   Modified TRV RNA2
   

   Use

   T-dna vector

   Mutation

   see comments section below

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

   Plasmid

   #71237

   Purpose

   Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A GFP
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFR WT

   Plasmid

   #11011

   Purpose

   Retroviral construct for expressing human EGFR in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-Cas9-Act3.0

   Plasmid

   #178954

   Purpose

   It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. QQR1 variant

   Plasmid

   #138564

   Purpose

   Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   QQR1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_OT1.0

   Plasmid

   #185386

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Has Service

   AAV1 and AAV9

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-BRD4-NUT
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6 and H1

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL-7xHis-TEV-hexa

    Plasmid

    #222868

    Purpose

    Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

    Depositor

    Nikolaus Loening

    Article

    Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

    Insert

    TEV protease
    

    Tags

    7xHis and MBP

    Expression

    Bacterial

    Mutation

    C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Promoter

    gpdA

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIN2 (gpdA-nTerm-mGL-ptrA)

    Plasmid

    #188736

    Purpose

    Expresses mGreenlantern for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-DIO-GRAB_OT1.0

    Plasmid

    #185387

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

    Plasmid

    #170229

    Purpose

    Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    Q64L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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