We narrowed to 50,410 results for: ANG

1. pX330 p53

   Plasmid

   #59910

   Purpose

   pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting p53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   Alla Karpova, David Schaffer

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiGuide Blast

   Plasmid

   #199622

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330 Pten

   Plasmid

   #59909

   Purpose

   pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-HA-Nr4a1

   Plasmid

   #160941

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTT5-hSDC1

   Plasmid

   #52326

   Purpose

   expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R117Q mutation (R95Q according to numbering in Fi…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV2-CAG-3xNLS-vsfGFP-0

   Plasmid

   #191097

   Purpose

   To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-vsfGFP-0
   

   Use

   AAV

   Tags

   Green fluorescent protein bound to enhancer nanob…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MYH11-NanoLuc-2A-tdTomato

    Plasmid

    #132569

    Purpose

    Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    Human MYH11 knockin homology arms (MYH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMThy1.1_hDDX5_FL

    Plasmid

    #88873

    Purpose

    constitutive expression of DDX5 in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-LAMP1-FLAG

    Plasmid

    #71868

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-flag-hKDM4A-polyA

    Plasmid

    #101051

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330 Ctnnb1.1

    Plasmid

    #59911

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-flag-hKDM4A(H188A)-polyA

    Plasmid

    #101052

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOD2046-bwmDEG

    Plasmid

    #89367

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pmyo-3

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid

    #156421

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOD2048-csnDEG

    Plasmid

    #89368

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Posm-6

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only